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LPO and cell death labeling Cells derived from cell culture or from IEC isolation procedures were incubated with BODIPY 581/591 C11 or the surface labeling antibodies (see below) at 37 C in the dark for ten to 30 min in flow cytometry buffer (2% FCS, 2 mM EDTA in PBS)

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This corroborates with another recent study that reported sEH-dependent alleviation of social deficits and fear responses in ASD mice occurs through the restoration of dysregulated mTOR-related pathways [129]